Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 37 bp insertion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-PPP2R1B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PPP2R1B also known as Protein Phosphatase 2 Scaffold Subunit A beta isoform acts as a scaffold for the assembly of PP2A complexes. PPP2R1B has a molecular mass of approximately 66 kDa. The protein expresses widely across various tissues with notable presence in the brain heart and skeletal muscle. As a scaffold subunit PPP2R1B coordinates the structural integrity necessary for phosphatase activity by recruiting regulatory and catalytic subunits important to its function.
Biological function summary
PPP2R1B integrates into the PP2A holoenzyme complex a major serine/threonine phosphatase. This complex regulates numerous cellular processes including cell growth division and signal transduction. PPP2R1B plays a role in maintaining cellular homeostasis through its regulatory activities. By doing so it links to various signaling events some of which control oncogenes and tumor suppressors demonstrating the protein's influence over cell cycle regulation and apoptosis.
Pathways
PPP2R1B plays a significant role in the Raf/MEK/ERK signaling pathway and the PI3K/AKT pathway. Through these pathways PPP2R1B interacts with PDPK1 and AKT1 modulating their phosphorylation status and thereby affecting cell proliferation and survival. By influencing such pathways PPP2R1B helps determine cellular responses to external stimuli impacting processes like differentiation and metabolism that are vital for organismal function.
PPP2R1B links to cancer and Alzheimer's disease. Mutations or downregulation in PPP2R1B have been implicated in colorectal cancer where it alters the PP2A activity and allows unregulated cellular proliferation. In Alzheimer's disease PPP2R1B's interaction with tau protein goes awry contributing to neurofibrillary tangle formation. These connections highlight the importance of PPP2R1B in understanding the molecular basis of these diseases and potential therapeutic targets.
