Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-PPP2R5E, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PPP2R5E also known as B56 epsilon is a regulatory subunit of protein phosphatase 2A (PP2A). It has an approximate mass of 72 kDa. PPP2R5E expresses across various tissues including the brain heart and skeletal muscle. The subunit associates with the core PP2A enzyme influencing substrate specificity and activity of PP2A holoenzyme.
Biological function summary
PPP2R5E plays a role in regulating cell growth and division. It is a part of the PP2A holoenzyme complex acting as a critical modulatory component. By associating with the PP2A core enzyme PPP2R5E contributes to the dephosphorylation process of many substrates. These substrates are vital for maintaining cellular functions such as signal transduction and cell cycle progression.
Pathways
PPP2R5E takes part in cell signaling and apoptotic pathways. It has substantial involvement in the MAPK signaling pathway where it modulates the activity of related kinases. PPP2R5E interacts with proteins like RAF1 influencing the pathway's function. Additionally it also participates in the Wnt signaling pathway where its regulatory role impacts cellular differentiation and proliferation.
PPP2R5E shows links to cancer and neurodegenerative diseases. Alterations in its expression or function may lead to oncogenesis due to its role in controlling cell proliferation signals. The dysregulation of PP2A activity potentially through PPP2R5E has associations with Alzheimer’s disease. In cancer PPP2R5E interacts with proteins such as c-Myc which contributes to tumorigenesis and progression.
