Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-RABAC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRA1 protein also known as prenylated Rab acceptor 1 is involved in vesicular transport processes within the cell. This protein has a molecular mass of approximately 21 kDa and plays an important role in the trafficking of proteins bound for various destinations in the secretory pathway. PRA1 is widely expressed in many tissues reflecting its integral function in cellular transport mechanisms.
Biological function summary
PRA1 interacts with Rab GTPases which suggest its role in facilitating the docking or fusion of transport vesicles with target membranes. Although it does not form a complex on its own PRA1 acts as an effector anchoring prenylated Rab proteins. This interaction is essential for maintaining the fidelity and specificity of vesicle transport events necessary for proper cellular functioning.
Pathways
PRA1 participates in the regulation of the Rab GTPase cycle and is important in the endocytic and secretory pathways. It modulates the activity of proteins such as Rab1 and Rab5 that coordinate the movement of vesicles from the endoplasmic reticulum to the Golgi and from the Golgi to the plasma membrane. These pathways are fundamental for cellular logistics ensuring that proteins reach the intracellular or extracellular locations where they are needed.
PRA1 has been linked to neurodegenerative conditions like Alzheimer's disease possibly due to its involvement in vesicular transport and protein trafficking dysregulation. Alterations in PRA1 expression or function can affect proteins such as Rab proteins potentially contributing to the pathological process of such disorders. Another condition associated with PRA1 is Charcot-Marie-Tooth disease suggesting its importance in neuronal maintenance and integrity.
