Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-TXNL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein TXNL1 also known as thioredoxin-like-1 functions mechanically as a member of the thioredoxin family. It contains an active site with a redox-active disulfide bond which allows it to facilitate electron transfer reactions. TXNL1 has a molecular mass of approximately 32 kDa. This protein is expressed in various tissues with distinctive presence in the heart skeletal muscles and liver.
Biological function summary
TXNL1 plays a role in maintaining cellular redox homeostasis and regulates the oxidative stress response. It participates in the thioredoxin complex working alongside other thioredoxin family members to reduce oxidized proteins within cells. By restoring proteins to their functional state TXNL1 helps ensure proper cellular function and protects against damage from reactive oxygen species.
Pathways
The involvement of TXNL1 extends to key cellular signaling pathways including the Nrf2-mediated antioxidant pathway and the MAPK pathway. In the Nrf2 pathway TXNL1 impacts the regulation of antioxidant response elements supporting cell survival under stress conditions. Within the MAPK pathway it interacts with signaling proteins such as JNK modulating stress responses and apoptosis.
TXNL1 shows a connection to conditions like cardiovascular disease and neurodegenerative disorders. Its role in oxidative stress makes it relevant in cardiovascular disease where imbalance in redox homeostasis contributes to disease progression. In neurodegenerative disorders interactions of TXNL1 with proteins such as Nrf2 play part in modulating neuronal survival and apoptosis highlighting its potential influence on disease mechanisms.
