Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-BCL2L2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bcl2L2 also known as Bcl-w is a protein that functions as an anti-apoptotic member of the Bcl-2 family. It has a molecular weight of approximately 21 kDa. This protein is expressed in various tissues such as the brain testis and kidney suggesting it plays a significant role in these organs. Bcl2L2's expression pattern and structure contribute to its function in regulating apoptotic pathways by inhibiting cell death signals.
Biological function summary
The function of Bcl-w involves its role in promoting cell survival. It is part of a complex network of interactions within the Bcl-2 family. This protein family includes both pro-apoptotic and anti-apoptotic proteins and Bcl-w helps maintain the balance by impeding apoptosis. The protein localizes to the mitochondrial outer membrane where it sequesters pro-apoptotic members such as Bax and Bak preventing the release of cytochrome c an essential step in the apoptosis process.
Pathways
The Bcl-w protein integrates into the intrinsic mitochondrial apoptosis pathway. It interacts closely with other Bcl-2 family proteins to ensure cellular homeostasis. Important pathways involving Bcl-w include the mitochondrial or intrinsic apoptosis pathway and the PI3K/AKT signaling pathway both of which regulate cell survival. Related proteins include Bcl-2 Bcl-XL Bax and Bak. These proteins collectively modulate the apoptosis signaling and therefore influence cell fate decisions within tissues where Bcl-w is expressed.
Bcl-w has been implicated in cancer and neurodegenerative diseases due to its role in cell survival and apoptosis regulation. In certain cancers the overexpression of Bcl-w protein can lead to unchecked cell proliferation by avoiding apoptosis therefore promoting tumor growth. In neurodegenerative diseases such as Alzheimer's disease altered expression of Bcl-w may contribute to neuronal survival affecting disease progression. Proteins like Bax and Bak which promote apoptosis are often in regulatory interplay with Bcl-w in these conditions highlighting the delicate balance of pro-survival and pro-apoptotic forces in disease states.
