Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 28 bp deletion in exon9.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-L3MBTL3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
L3MBTL3 also known as Lethal(3) Malignant Brain Tumor-Like Protein 3 is a member of the polycomb group proteins which regulate gene expression by remodeling chromatin. It has a molecular weight of approximately 69 kDa. L3MBTL3 is widely expressed in various tissues with significant levels in the brain and hematopoietic cells. This protein plays a role in chromatin compaction through its ability to recognize and bind mono- and dimethylated lysine residues on histones. These activity patterns classify it as a histone reader influencing the accessibility of transcriptionally active regions.
Biological function summary
L3MBTL3 affects cellular processes by managing chromatin structure which in turn regulates gene expression. It participates in chromatin remodeling as part of large multi-protein complexes. By interacting with different components L3MBTL3 modulates the transcription of oncogenes and tumor suppressors. It contributes to important cellular processes including cell-cycle progression and differentiation by establishing particular transcriptional profiles. The protein's role in these processes highlights its importance in maintaining normal cell function.
Pathways
L3MBTL3 participates in pathways that involve chromatin modification and gene silencing. It is associated with the Polycomb Repressive Complex 2 (PRC2) pathway which plays a role in maintaining transcriptional repression. This pathway involves key proteins such as EZH2 and SUZ12 which work alongside L3MBTL3 to modify histone marks thereby silencing target genes. This positioning within pathways makes it an integral component of transcriptional regulation by ensuring genes are restricted as necessary for proper development and cellular activity.
L3MBTL3 has associations with cancer and hematological abnormalities. Its dysregulation has links to certain types of leukemia where abnormal expression can lead to uncontrolled cell proliferation. Another condition tied to L3MBTL3 is brain malignancies due to perturbations in its normal function within neuronal cells. Interactions with proteins like EZH2 further connect L3MBTL3 to cancer pathways emphasizing the relevance of its careful regulation in maintaining cellular health and preventing disease.
