Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-PDCD6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDCD6 also known as ALG-2 is a protein with a molecular mass of about 22 kDa. It is expressed widely in human tissues with high levels observed in the thymus and spleen. The protein acts as a calcium-binding molecule interacting with other proteins in a calcium-dependent manner. PDCD6 has alternate names including ALG-15 and ALG-30 referencing its expression in certain cell types and conditions. The protein's role as a mediator in apoptotic cell death is well-known making it of significant interest to those studying cell death mechanisms.
Biological function summary
The function of PDCD6 involves its participation in calcium signaling pathways. This protein is part of a larger complex involving T-cell receptor signaling and also influences the Fas ligand-mediated apoptosis pathway. PDCD6 achieves its regulatory roles through interactions with various proteins such as MA3Ps. As a calcium-binding protein ALG-2 modulates calcium homeostasis which is critical for maintaining cellular functions including cell proliferation differentiation and programmed cell death.
Pathways
The involvement of PDCD6 in apoptosis is significant. It works in coordination with pathway proteins such as caspases and fas-associated proteins influencing cell death signaling. In addition PDCD6's ability to bind calcium allows it to fit into the broader intracellular signaling network placing it in connection with MAPK pathways essential for cellular responses to stress and growth signals. ALG protein interactions underline PDCD6's influence across apoptosis and signaling pathways.
PDCD6 links to pathological states like cancer and neurodegenerative disorders. Increased expression or altered function can impact apoptosis leading to uncontrolled cell growth in cancers or heightened cell death in neurodegenerative conditions. Additionally PDCD6 shows associations with proteins like anti-ALG and ALG protein variants influencing pathological processes in lymphomas and Alzheimer's disease. Understanding PDCD6's role gives insights into therapeutic strategies targeting calcium-dependent signaling and apoptotic regulation.
