Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 23 bp deletion in exon 2 and 94 bp deletion in exon 2,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-EMC6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Transmembrane protein 93 also known as EMC6 functions as a membrane protein in intracellular transport and protein folding. EMC6 is part of the endoplasmic reticulum membrane protein complex (EMC) and has a molecular mass of approximately 26 kDa. It expresses widely in various tissues including brain heart liver and kidney suggesting a broad role in cellular processes.
Biological function summary
This protein interacts with other components in the endoplasmic reticulum and is essential for proper folding and insertion of proteins into the ER membrane. EMC6 functions as part of a larger scaffolding complex that supports ER-associated degradation (ERAD) which is necessary for maintaining protein homeostasis. This complex plays an important role in quality control by removing misfolded proteins and EMC6 specifically contributes to the stabilization and function of this complex.
Pathways
EMC6 has important roles in the ER stress response and autophagy pathways. The protein interacts with autophagy-related proteins including Beclin 1 facilitating the cellular recycling process. Through the ER stress response EMC6 participates in maintaining cellular health by assisting in protein refolding and degradation pathways linking its function to the action of other ER stress-related proteins like IRE1 and PERK.
EMC6 associates with conditions like neurodegenerative diseases and certain cancers. The protein's involvement in autophagy links it to Alzheimer's disease where accumulation of defective proteins occurs. Additionally disruptions in EMC6 function may contribute to oncogenesis by impairing protein quality control with its role in ERAD connecting it to the growth of cancerous cells. Altered expression or function of EMC6 can correlate with disease pathogenesis making it a point of interest for therapeutic research.
