Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-LAMTOR4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMTOR4 also known as late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 is a component of the Ragulator complex. It interacts with specific proteins to anchor the Rag GTPases on lysosomal membranes. LAMTOR4 is a protein with a mass of approximately 11 kDa. Researchers have detected its expression primarily in cellular membranes especially within lysosomes positioning it as key in related processes.
Biological function summary
This protein plays a role in nutrient sensing and cellular growth regulation. LAMTOR4 is part of the Ragulator complex a scaffolding complex essential for the activation of mTORC1 a central regulator of cell growth and metabolism. LAMTOR4 contributes to the recruitment and activation of mTORC1 at the lysosomal surface which is important for the integration of nutrient and energetic signals.
Pathways
LAMTOR4 is involved in the mTOR signaling pathway closely related to cellular metabolism and growth. This protein interacts with other members of the Ragulator complex like LAMTOR1 and LAMTOR2 all connecting in the process of mTORC1 activation. Through the mTOR pathway LAMTOR4 modulates autophagy and anabolic processes therefore maintaining cellular homeostasis.
Alterations in the function of LAMTOR4 have associations with metabolic disorders and cancerous conditions. The regulatory role of LAMTOR4 in mTORC1 activation links it to conditions such as tuberous sclerosis complex where mTOR signaling is disrupted. Alterations in LAMTOR4 and associated proteins like mTOR and other Ragulator complex members can contribute to pathogenesis by disrupting normal cellular growth and metabolism.
