Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-SH3GL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EEN also known as Endophilin A2 is a protein involved in endocytosis and membrane trafficking. EEN has a molecular weight of approximately 44 kDa and is mentioned in several tissues including the brain where it shows high expression levels. This protein aids in clathrin-mediated endocytosis by binding to lipids and proteins playing a critical role in membrane dynamics. EEN interacts with SH3 domain-containing proteins contributing to its function in cellular processes.
Biological function summary
EEN participates in the formation of protein complexes necessary for synaptic vesicle recycling important for neurotransmitter release in neurons. It binds to proline-rich motifs on its partner proteins which helps in vesicle scission during endocytosis. These interactions support vesicle fission and transport making EEN important for maintaining efficient synaptic transmission. Apart from neurons EEN also contributes to endocytic processes in other cell types indicating its wider functional role.
Pathways
EEN plays a significant role in the regulation of intracellular trafficking pathways and is a part of the endocytic pathway. It links with proteins such as dynamin and clathrin integral components of the endocytic machinery that facilitate vesicle budding and release. These interactions ensure proper recycling of synaptic vesicles and membrane components vital for cellular homeostasis. Collaborations with proteins like Bin1 show EEN's involvement in coordinating endocytosis and membrane curvature recognition.
EEN associates with conditions related to neurological function and cancer development. Aberrant expression of EEN contributes to certain cancers where disturbances in endocytosis and signaling pathways can lead to uncontrolled cell growth. The interaction with proteins such as c-Cbl an E3 ubiquitin-protein ligase implicates EEN in pathways relevant to tumor progression. Additionally altered EEN function might impact neurodegenerative diseases stemming from its contribution to synaptic vesicle trafficking reflecting the importance of its proper regulation in maintaining cell functions.
