Abcam, ab26-20µg, Anti-p53 antibody [PAb 240]

ab26 has been knockout validated in Western blot. The expected band was seen in wild type HCT116 cells treated with the DNA damaging agent irinotecan and no band was seen in TP53 knockout HCT116 cells.
Product Specifications Anti-p53 antibody [PAb 240] (ab26) is a mouse monoclonal antibody and is validated for use in ICC/IF, IP, WB in human, mouse samples. Anti-p53 antibody [PAb 240] (ab26) specifically detects p53 (UniProt ID: P04637; Molecular weight: 44kDa) and is sold in 100 µg selling sizes. Quality and Validation Abcam's high quality validation processes ensure Anti-p53 antibody [PAb 240] (ab26) has high sensitivity and specificity. The specificity of Anti-p53 antibody [PAb 240] (ab26) has been confirmed by testing in knockout samples. Anti-p53 antibody [PAb 240] (ab26) has been cited over 403 times in peer reviewed journals and is trusted by the scientific community. Anti-p53 antibody [PAb 240] (ab26) has 54 independent reviews from customers. Related Products Conjugation-ready, carrier free format available for antibody clone PAb 240 - ab185238.
Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.