Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 504 pg/mL,
Range:615 - 7000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Alpha-synuclein ELISA Kit ab260052 is a rapid single-wash 90-min ELISA kit to measure Human Alpha-synuclein in Tissue lysate, cell lysate, cell culture media, cell culture supernatant, plasma and serum. This SimpleStep ELISA® sensitivity is 504 pg/mL.
How the assay works
Human Alpha-synuclein SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human human α-Synuclein protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only. Urine, saliva, and cerebrospinal fluid samples have not been tested with this kit. Both monomeric and aggregated Alpha-synuclein can be detected with this kit.
Human Alpha-synuclein ELISA Kit ab260052 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Alpha-synuclein ELISA kit ab260052
Human Alpha-synuclein ELISA kit ab260052 has been used in publications to study a targeted protein degradation platform for developing drugs to delay symptoms of Parkinson's Disease
, the correlation of alpha-synuclein levels with neuronal thread protein and apolipoprotein E in Alzheimer's Disease
, and metabolic reprogramming of misfolded proteins in neurodegenerative disorders using polyphenol
References:
Young Ho Suh et al. 2023 PMID: 37355598,
Dantao Peng et al. 2021 PMID: 34312362,
Hongzhuan Chen et al. 2023 PMID: 36873190
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Alpha-synuclein Antibody Pair - BSA and Azide free
ab270346
- Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture)
ab259611
- Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (Capture/Detector)
ab259612
Related and recommended products
Human Alpha-synuclein ELISA kit ab260052 is commonly used to study neurodegenerative diseases such as Parkinson's disease and Lewy body dementia.
Other kits for measuring neurodegeneration include:
- Human IL-1 beta ELISA kit
ab214025
- Human Amyloid beta 1-40 ELISA kit
ab193692
- Human Amyloid Beta 42 ELISA kit
ab289832
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
