Description
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 1 bp deletion
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-PHF6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHD finger protein 6 also known as PHF6 is a protein with a molecular mass of approximately 43 kDa. It consists of two PHD domains which are zinc finger-like motifs playing roles in chromatin remodeling. PHF6 expresses widely in various tissues with noticeable levels in the brain and hematopoietic cells indicating its significance in these areas. Alternate names for PHF6 include ZNF312 and BORJ although PHF6 is the most commonly referred designation.
Biological function summary
PHF6 involves a range of cellular processes including regulation of transcription and chromatin structure modification. PHF6 can bind to certain DNA sequences and proteins to influence gene expression. It interacts with chromatin remodeling complexes indicating a role in maintaining chromatin accessibility for transcription factors. The protein's PHD domains contribute to its ability to engage in these interactions impacting the transcriptional activity of target genes.
Pathways
PHF6 interacts with transcriptional and chromatin remodeling pathways. It plays a role in the interplay between chromatin and transcriptional machinery which is critical for regulating gene expression. PHF6 associates with proteins such as nucleophosmin and other chromatin regulators influencing pathways like the Notch signaling pathway which is vital for cellular differentiation and development. These interactions highlight its involvement in genetic regulation within developmental pathways.
Mutations in PHF6 link to Börjeson-Forssman-Lehmann syndrome (BFLS) a rare genetic disorder affecting neurological development. Additionally PHF6 mutations recurrently appear in T-cell acute lymphoblastic leukemia (T-ALL) highlighting a role in hematological malignancies. In the context of T-ALL PHF6 interacts with proteins like Notch1 which drives leukemic proliferation when dysregulated. These associations make PHF6 a significant focus for understanding and potentially targeting specific genetic disorders and cancers.
