Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-BZW1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BZW1 also known as Protein Orf is a member of the basic leucine zipper (bZIP) family of proteins. The mass of BZW1 is approximately 42 kDa. It is mainly expressed in the nucleus of cells. BZW1 plays a role in the regulation of transcription and translation processes. This regulation occurs through its interaction with specific sequences on the DNA and RNA contributing to the modification of gene expression.
Biological function summary
BZW1 influences cell growth and proliferation by engaging in the regulation of gene transcription. It can interact with other proteins to form complexes that modulate these processes. BZW1 adjusts protein synthesis rates by acting at the level of translational control affecting cellular responses to different growth conditions. Its function supports overall cellular homeostasis by ensuring proteins are produced as needed.
Pathways
BZW1 is integral to both the mTOR and eIF2 signaling pathways which are critical in regulating protein synthesis and stress responses. It is related to other proteins like mTOR and eIF2α within these pathways and its activity can influence how cells respond to nutrient availability and environmental changes.
Alterations in BZW1 expression have associations with cancer and neurodegenerative diseases. In cancer dysregulation of BZW1 can affect the cell cycle by impacting the proteins involved like p53 thereby promoting uncontrolled cell proliferation. In neurodegenerative diseases changes in its function can disrupt protein homeostasis leading to the accumulation of proteins that contribute to disease progression and pathways involving proteins like α-synuclein.
