Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CMC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C16orf61 also known as Chromosome 16 Open Reading Frame 61 is a protein coded by the C16orf61 gene. It has a molecular mass of approximately 14 kDa. This protein is expressed in many tissues including the liver kidney and brain indicating its widespread role in cellular processes. Its exact mechanical function remains undetermined though researchers continue to explore its role in various cellular contexts.
Biological function summary
C16orf61 appears to have a significant role in cell cycle regulation and cellular growth. It does not currently belong to any known protein complexes but experimental data suggest it might interact with other proteins involved in growth regulation. Active studies investigate how C16orf61 may influence cellular homeostasis and proliferation.
Pathways
Researchers suggest that C16orf61 might interact with the PI3K/AKT signaling pathway an important pathway for cell survival and growth. This protein may also intersect with the Wnt signaling pathway impacting cell fate and embryonic development. Interactions with these pathways indicate potential connections with proteins like AKT1 and beta-catenin highlighting its possible involvement in broader signaling networks.
Studies indicate potential links between C16orf61 and cancer given its role in growth regulation pathways. C16orf61 may influence tumor development or progression through its interactions with proteins like PTEN in the PI3K/AKT pathway. Additionally there is a plausible association with neurodevelopmental disorders potentially through its involvement in the Wnt signaling pathway although this requires further validation.
