Key facts
Reacts with:Human
Product details:
HIF-1 alpha (H1alpha67, EP1215Y, ESEE122, EPR16897) Antibody Panel ab263460 contains multiple trial-sized versions of anti-human antibody clones against HIF-1 alpha, specifically selected for high performance in various applications. This panel contains 2 recombinant rabbit monoclonal antibodies and 2 mouse monoclonal antibodies against human HIF-1 alpha. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Mouse monoclonal [H1alpha67] to HIF-1 alpha (20 μg)
- Rabbit monoclonal [EP1215Y] to HIF-1 alpha (20 μL)
ab51608
- Mouse monoclonal [ESEE122] to HIF-1 alpha (20 μg)
ab8366
- Rabbit monoclonal [EPR16897] to HIF-1 alpha (20 μL)
ab179483
HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia. Under such conditions, it can activate over 40 genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. (
SwissProt: Q16665
). Under normoxic conditions, HIF-1 alpha is largely undetectable and hypoxia will need to be induced in most cell lines and tissues before testing. Induction is typically not required for cancerous tissues where hypoxic regions are common within the tumor microenvironment (PubMed: 1850121, 11689469).
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email
[email protected].
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
