Key facts
Reacts with:Human,
Target:LMNASee target data
Product details:
Nuclear Marker (Lamin A + C, SC35, KDM1/LSD1, Fibrillarin, HP1 alpha) Antibody Sampler Panel ab263467 contains multiple trial-sized versions of anti-human antibody clones against Lamin A + C, SC35, KDM1/LSD1, Fibrillarin and HP1 alpha specifically selected for high performance in various applications. This panel contains 4 recombinant rabbit monoclonal antibodies against Lamin A + C, KDM1/LSD1, Fibrillarin and HP1 alpha and 2 mouse monoclonal antibody against Lamin A + C and SC35. Most of the clones in this panel also react with mouse and rat (please refer to each individual datasheet). They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR4100] to Lamin A + C - Nuclear Envelope Marker (20 μL)
ab108595
- Mouse monoclonal [4C11] to Lamin A + C (20 μg)
ab238303
- Mouse monoclonal [SC-35] to SC35 - Nuclear Speckle Marker (20 μg)
ab11826
- Rabbit monoclonal [EPR6825] to KDM1 / LSD1 - Nuclear Marker (20 μL)
ab129195
- Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker (20 μL)
ab166630
- Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker (20 μL)
ab109028
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email
[email protected].
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HP1 alpha also known as Heterochromatin Protein 1 Alpha is a chromatin-associated protein involved in modifying the structural organization of chromatin. It has a mass of around 22 to 25 kDa and predominantly expresses in the nucleus. HP1 alpha interacts with histone H3 through methylation marks at lysine 9 contributing to chromatin compaction and epigenetic regulation. Fibrillarin with an estimated mass of 34 kDa is a nucleolar protein involved in rRNA processing and a component of small nucleolar ribonucleoproteins (snoRNPs). Lamin A and Lamin C splice variants with molecular masses of about 74 kDa and 65 kDa respectively are intermediate filament proteins forming nuclear lamina essential for maintaining nuclear envelope integrity. SC35 also known as SRSF2 is part of the splicing machinery within the nuclear speckles. KDM1/LSD1 (Lysine-specific demethylase 1) is expressed mainly in the nucleus as well with a mass of approximately 95 kDa.
Biological function summary
HP1 alpha plays a significant role in gene silencing and chromatin remodeling. It often forms complexes with other regulatory proteins like KAP1 and SUV39H1 to influence transcriptional repression. Fibrillarin is important in ribosome biogenesis pivotal for cell growth and proliferation. Lamin A and Lamin C form networks critical in organizing nuclear architecture and regulate processes such as DNA replication and transcription through their scaffold-like structure. SC35 is integral in mRNA splicing and also impacts alternative splicing impacting gene expression regulation. KDM1/LSD1 acts as a demethylase influencing gene expression by removing methyl groups from histone marks affecting cell differentiation and development.
Pathways
HP1 alpha integrates into chromatin modification pathways influencing transcriptional regulation through interactions with proteins such as histone deacetylases. Fibrillarin actively participates in ribosomal RNA processing pathways affecting ribosome assembly and protein synthesis capacity of the cell. Lamin A and Lamin C are linked to cell cycle progression pathways ensuring proper nuclear shape and size. SC35 has important roles in the splicing pathways affecting RNA processing and subsequently impacting gene expression levels. KDM1/LSD1 functions within epigenetic regulation pathways which control gene expression patterns through histone modification.
Mutations or dysregulation involving Lamin A/C can result in laminopathies such as muscular dystrophy and Hutchinson-Gilford Progeria Syndrome. Lamin A/C's integral role in nuclear stability connects it to these structural disorders. Abnormal KDM1/LSD1 activity links to neurodegenerative diseases due to its role in chromatin remodeling and transcription regulation. SC35 misregulation has associations with some cancers influencing tumor behavior by affecting splicing. Increased expression of HP1 alpha correlates with certain aggressive cancer types due to its impact on chromatin state and gene silencing interacting with key oncogenes.
