Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-EPHA4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Eph receptor A4 also known as SEK is a type of ephrin receptor that plays a significant role in cellular communication. It belongs to the Eph protein family and it is classified as a receptor tyrosine kinase. EphA4 has an approximate molecular mass of 110 kDa. It is mainly expressed in the nervous system although it can also be found in other tissues including the vascular and skeletal systems. This receptor has an important function in mediating signal transduction upon binding with their ephrin ligands.
Biological function summary
EphA4 plays a critical role in developmental processes particularly in the development of the nervous system and vascular patterning. The receptor engages in bidirectional signaling meaning that signals are transmitted not only into the receptor-expressing cells but also into the ephrin-expressing ones. EphA4 is part of complexes that can include multiple receptor and ephrin molecules which allows for the modulation of cell adhesion and migration. These processes are essential for forming tissue boundaries and axon guidance.
Pathways
EphA4 signaling is involved in two significant pathways: the Eph/ephrin signaling pathway and the cytoskeletal remodeling pathway. In the Eph/ephrin signaling pathway EphA4 interacts with various ephrin ligands leading to changes in cellular architecture and behavior. The cytoskeletal remodeling pathway involves interactions with proteins such as Rho and Ras which coordinate cytoskeletal changes necessary for cell movement and positioning. These pathways are integral to processes like axon guidance and cell migration underpinning EphA4's role in development.
EphA4 has been implicated in conditions such as amyotrophic lateral sclerosis (ALS) and cancer metastasis. In the context of ALS EphA4's interaction with proteins like RhoA affects axonal repair and neuron survival contributing to the disease's progression. Regarding cancer EphA4 influences tumor growth and spread with its signaling pathways impacting cellular invasion and angiogenesis. Understanding these aspects of EphA4 activity assists in developing therapeutic strategies for these disorders.
