Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hairless also known as HR is a nuclear receptor corepressor that plays an important role in hair follicle development. With a molecular mass of approximately 130 kDa it acts mechanistically by interacting with transcription factors to regulate gene expression. Hairless is expressed mainly in the skin particularly in the hair follicles but it can also be found in other tissues like the brain adipose tissue and testes. Its ability to localize in the nucleus allows Hairless to directly control chromatin structure and transcriptional activity.
Biological function summary
Hairless is involved in the regulation of hair cycling and differentiation. It functions as part of a larger repressor complex recruiting histone deacetylases to histones which results in gene silencing. This activity is important for the transition between different phases of the hair growth cycle such as anagen and telogen phases. Hairless also plays a role in repressing certain genes involved in epidermal differentiation thereby maintaining stem cell populations necessary for continuous hair growth.
Pathways
Hairless significantly impacts the Wnt signaling pathway and the Notch signaling pathway. In the Wnt pathway Hairless collaborates with proteins like beta-catenin to influence follicle morphogenesis and hair cycle progression. In the Notch signaling cascade Hairless interacts with Notch receptors regulating cell fate decisions during hair development. These pathways are essential for proper hair follicle regeneration and maintenance highlighting Hairless's critical role in hair biology.
Hairless has been implicated in alopecia universalis a condition characterized by complete hair loss. Mutations in the Hairless gene can result in faulty protein function causing disruption in hair development and cycling. Furthermore Hairless has ties to Marie Unna hereditary hypotrichosis a genetic disorder leading to sparse hair and scalp abnormalities. The implicated pathways also involve proteins such as Notch1 and beta-catenin which are associated with defects in hair follicle development and structure.
