Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 3 and 95 bp deletion in exon 3,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-EML4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EML4 also known as Echinoderm Microtubule-Associated Protein-Like 4 is a microtubule-associated protein with a mass of approximately 120 kDa. This protein stabilizes microtubules and influences their organization during cell division. EML4 is widely expressed in various tissues particularly in the brain where it plays important roles in neuronal function. It interacts with microtubule structures to ensure proper chromosome segregation and mitotic spindle formation.
Biological function summary
EML4 facilitates microtubule stability and dynamics which are critical for intracellular transport and cell division. It is part of the EML family and can form complexes with other microtubule-associated proteins. EML4 helps in the regulation of structural arrangements that are important in both mitosis and meiosis processes. Its function in axonal transport becomes essential for proper neuronal development and maintenance.
Pathways
EML4 is involved in the MAPK/ERK and Ras signaling pathways. Within these pathways EML4 interacts with key proteins like KRAS and BRAF contributing to signal transduction that controls cell proliferation and differentiation. The protein's regulatory role in microtubule dynamics positions it as a link between cellular signaling and cytoskeletal organization.
EML4 is most commonly associated with non-small cell lung cancer (NSCLC). A gene fusion between EML4 and ALK (anaplastic lymphoma kinase) leads to the production of an oncogenic protein that drives tumorigenesis. This EML4-ALK fusion protein alters normal cell signaling pathways promoting cancer cell survival and growth. Research also connects EML4 to neurodegenerative diseases due to its important role in microtubule stabilization within neurons.
