Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 2 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CAPG, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Actin Regulatory Protein CAPG also known as MCP is an important cytoskeletal protein involved in actin filament dynamics. Other common names include CAPG protein and capping proteins. CAPG binds to the barbed ends of actin filaments modulating their length and polymerization. This protein has a molecular weight of approximately 39 kDa. It is expressed in various tissues with significant expression in HeLa cells which are often used in studies related to actin regulation.
Biological function summary
This actin regulatory protein serves as an important player in the remodeling of the actin cytoskeleton. CAPG functions by capping the fast-growing ends of actin filaments which influences cell motility and structure. It is not known to be part of a larger protein complex but interacts closely with other actin-modulating proteins. MCP's regulation of cytoskeletal dynamics allows cells to respond swiftly to environmental changes assisting cellular processes like migration and secretion.
Pathways
CAPG is intricately involved in pathways controlling cell motility and structure including the cytoskeletal rearrangement pathway. It works alongside proteins such as cofilin and Arp2/3 complex which also regulate actin dynamics. These proteins together facilitate processes like cell division and migration making their interactions essential for proper cellular function.
CAPG's regulation is pertinent in cancer metastasis particularly in its relation to HeLa cells used in cancer studies. It affects tumor cell invasiveness by altering the cytoskeleton connecting it to processes involving other regulatory proteins like Rac1. Additionally CAPG plays a role in neurodegenerative disorders by impacting neuronal cell motility where interactions with actin-modulating proteins are important. Understanding CAPG's role gives insight into potential interventions for these disorders.
