Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CPSF7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CPSF7 also known as cleavage and polyadenylation specificity factor subunit 7 is a protein that participates in mRNA processing. With a mass of approximately 55 kDa CPSF7 resides in the nucleus of cells where it plays a role in the cleavage and polyadenylation of pre-mRNA. This protein is expressed in various tissues emphasizing its broad role in gene expression regulation across different cell types.
Biological function summary
CPSF7 functions in the formation of the cleavage and polyadenylation complex which is essential for proper mRNA maturation. It interacts with other subunits in this complex to ensure accurate mRNA 3' end processing. This protein participates in determining the polyadenylation site a critical step that influences mRNA stability export and translation.
Pathways
CPSF7 integrates into the post-transcriptional regulation of gene expression. It plays important roles within the mRNA processing pathway and is linked to the regulation of the cell cycle. CPSF7 interacts with other proteins such as CPSF3 within these pathways to mediate these biological processes that are pivotal for efficient gene expression and cell function.
Aberrations in CPSF7 function relate to cancer and autoimmune diseases. In cancer altered expression of CPSF7 may affect tumor growth and progression potentially through interactions with other proteins like CPSF73. Autoimmune diseases could also involve CPSF7 due to disruptions in mRNA processing which might affect immune cell function and regulation.
