Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 5 bp deletion in exon 2
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ABCF2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The human ABCF2 protein also known as ATP-binding cassette sub-family F member 2 belongs to the ABC transporter superfamily. Weighing approximately 85 kDa ABCF2 does not transport molecules across membranes like most other members of this family. Instead it participates in regulating translation and is involved in cellular stress responses. Researchers find ABCF2 expression in various tissues including liver lung and muscle. It does not span membranes and it anchors within the cytoplasm of cells.
Biological function summary
ABCF2 impacts translation regulation and stress response through different cellular processes. This protein functions independently and may also participate in complexes with other proteins. Through mechanisms not fully understood ABCF2 associates with cellular stress granules that form in response to environmental stress. These interactions suggest roles in protein synthesis regulation during stress which indirectly influences cell survival and adaptation.
Pathways
ABCF2 integrates into cellular processes related to stress and translation. It intersects with pathways like eukaryotic initiation factor 2 (EIF2) signaling which helps control translation initiation during stress. ABCF2 shares pathway interactions with proteins such as EIF2B and other ribosomal elements indicating a connection to translation regulation. The involvement in ribosomal function places ABCF2 within important cellular response networks especially under conditions that affect protein synthesis fidelity.
ABCF2 links to specific pathologies primarily through stress response mechanisms. Cancer particularly hepatic and pulmonary types shows associations with dysregulated ABCF2 expression possibly affecting tumor growth and chemoresistance. Researchers note potential interactions with heat shock proteins which share pathways implicated in cancer cell survival. Additionally preliminary evidence suggests ABCF2 roles in neurological disorders although the connections and interactions with other proteins need further study.
