Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-COX8A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
COX8A also known as Cytochrome c oxidase subunit 8A is a component of the cytochrome c oxidase complex which plays a vital role in the electron transport chain within mitochondria. This protein has a molecular mass of approximately 8.7 kDa. COX8A is highly expressed in tissues with high energy demands such as muscle and brain where efficient ATP production is needed.
Biological function summary
COX8A is an integral part of the large cytochrome c oxidase complex also known as Complex IV in the mitochondrial inner membrane. This complex is involved in oxidative phosphorylation where it catalyzes the reduction of oxygen to water a final step of the electron transport chain. COX8A contributes to the correct assembly and stability of the cytochrome c oxidase complex ensuring proper mitochondrial function and energy production.
Pathways
COX8A participates in the oxidative phosphorylation pathway and is an important player in the process that produces ATP from ADP and inorganic phosphate. It functions together with other subunits in Complex IV such as COX2 and COX3 to facilitate efficient electron transfer and proton pumping. Through its activity in these processes COX8A directly influences cellular respiration and energy metabolism which are critical for cellular homeostasis.
COX8A has connections to mitochondrial disorders such as Leigh syndrome and cytochrome c oxidase deficiency. These conditions arise from defects in mitochondrial oxidative phosphorylation affecting tissues with high metabolic demands. Alterations in COX8A along with other complex IV components like COX2 can contribute to the pathogenesis of these diseases as they disrupt ATP production and lead to energy deficits in affected cells.
