Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts, Cell Lysate,
Reacts with:Mouse, Rat, Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 38.39 pg/mL,
Range:203.13 - 13000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
DSCAM ELISA Kit (ab269543) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DSCAM protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate DSCAM with 38.39 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
DSCAM is an adhesion protein expressed in the highest level in the fetal brain, although DSCAM is also expressed in other tissues and in adults. DSCAM is involved in neurogenesis and self-avoidance, but also in immunity in invertebrates. Overexpression of DSCAM in the fetal brain leads to Down syndrome, and altered DSCAM levels have been observed in PTSD, bipolar disorder, depression and Alzheimer disease. DSCAM has high amino acid sequence identity between human, mouse and rat. The standard protein in this product is human DSCAM.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dscam known as Down Syndrome Cell Adhesion Molecule is a protein with a mass of around 230 kDa. It functions as a cell adhesion molecule and is involved in axon guidance. Dscam is expressed in various tissues with high levels in the nervous system particularly during developmental stages. The protein plays a role in neural development by ensuring proper neuronal connections.
Biological function summary
Dscam contributes to neuronal differentiation and synaptic targeting. It belongs to the immunoglobulin superfamily and is involved in forming non-covalent complexes with other cell adhesion molecules. The protein facilitates homophilic binding which regulates axonal and dendritic branching. This process ensures neural circuits form correctly impacting brain development and function.
Pathways
Dscam participates in the neural development pathway which includes interactions with the Robo receptor affecting axonal migration and guidance. The protein also links to the slit-Robo pathway playing a substantial role in neural patterning. These interactions ensure the precise wiring required for functional neural networks influencing how neural signals propagate.
Dscam's dysregulation connects to neurological conditions like Down syndrome and autism spectrum disorders. It interacts with proteins involved in these disorders like the ROBO proteins which mediate axon guidance. Accumulating evidence suggests that altered Dscam expression impairs neuronal connectivity contributing to the pathophysiology of these conditions.
