Description
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Next Generation Sequencing,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 19 bp deletion Frameshift = 99.67%
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-SIRT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIRT1 also known as Silent mating type information regulation 2 homolog 1 is a NAD-dependent deacetylase enzyme. SIRT1 weighs approximately 120 kDa and plays an important role in regulating transcription apoptosis and stress resistance. Researchers have found SIRT1 in various tissues with higher expression in the heart brain and skeletal muscle. It is a component of the larger family of sirtuins which are involved in metabolic regulation and aging.
Biological function summary
SIRT1 modulates several cellular processes such as gene silencing DNA repair and lifespan extension. SIRT1 participates in complexes with other proteins including histones and transcription factors to influence chromatin structure and gene expression. It acts through deacetylation of target proteins affecting their function and stability. The activity of SIRT1 is also linked to environmental and cellular conditions including caloric intake and oxidative stress.
Pathways
SIRT1 is integral in the regulation of metabolic and longevity pathways. It interacts with the FOXO family proteins and the tumor suppressor protein p53 aiding in response to cellular stress and metabolic demands. The role of SIRT1 in the insulin signaling pathway exemplifies its influence on glucose homeostasis and energy balance. These interactions highlight its importance in metabolic health and aging.
SIRT1 links to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like type 2 diabetes. In Alzheimer's disease SIRT1 interacts with the amyloid precursor protein suggesting a protective role against amyloid-beta accumulation. Additionally studies have shown connections between SIRT1 and insulin receptor substrates highlighting its role in managing insulin sensitivity and glucose metabolism in diabetes. Understanding SIRT1's functions offers potential therapeutic targets for these disorders.
