Key facts
Reacts with:Human, Mouse, Rat,
Target:ATG16L1See target data,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.
Product details:
Please note that Recombinant Anti-ATG16L1 (phospho S278) antibody [EPR19016] is for mouse and human reactivity only. The rest of the clones in this panel react with human, mouse, and rat.
Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel ab269811 contains multiple trial-sized versions of anti-human, mouse and rat antibody clones against ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin and M6PR, specifically selected for high performance in in various applications. This panel contains 6 recombinant rabbit monoclonal antibodies against human, mouse and rat ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin and M6PR. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
Recombinant Anti-ATG16L1 (phospho S278) antibody [EPR19016] (20 μL)
ab195242
Recombinant Anti-ATG16L1 antibody [EPR15638] - N-terminal antibody (20 μL)
ab187671
Recombinant Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker antibody (20 μL)
ab109012
Recombinant Anti-LC3B antibody [EPR18709] - Autophagosome Marker antibody (20 μL)
ab192890
Recombinant Anti-Ubiquitin antibody [EPR8830] (20 μL)
ab134953
Recombinant Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (20 μL)
ab124767
Please note that Recombinant Anti-ATG16L1 (phospho S278) antibody [EPR19016] is for mouse reactivity only. The rest of the clones in this panel react with human, mouse, and rat.
For optimal WB results using
ab195242
, we recommend blocking with 10X Blocking Buffer (ab126587)
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email
[email protected].
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ubiquitin acts as a small regulatory protein with a mass of around 8.5 kDa. It is widely expressed in eukaryotic cells and involved in tagging cellular proteins for degradation. M6PR or cation-independent mannose-6-phosphate receptor functions in the lysosomal targeting of proteins aiding in lysosomal enzyme transport. SQSTM1 commonly known as p62 plays an important role in autophagy by linking ubiquitinated proteins to the autophagy machinery. ATG16L1 essential for autophagy participates in the formation of autophagosomes. LC3B a marker of autophagy associates with autophagosomes and its lipidation signifies autophagic activity.
Biological function summary
These proteins serve central roles in cellular homeostasis and degradation processes. Ubiquitin is integral in tagging proteins for proteasomal degradation. M6PR functions within lysosomal enzyme trafficking while p62 aggregates ubiquitinated proteins for autophagic degradation acting as a bridge to autophagic cargo. ATG16L1 stands as part of the autophagy-related gene complex important for the elongation of the autophagosome membrane. LC3B when lipidated embeds into autophagosome membranes distinguishing active autophagy processes.
Pathways
These proteins integrate into the ubiquitin-proteasome and autophagy-lysosome systems. Ubiquitin interacts with various cellular pathways to manage protein turnover and degradation. The recycling of cellular components through autophagy involves p62 and ATG16L1 where LC3B serves as an autophagosome marker. These pathways intersect with cellular stress responses involving other proteins like mTOR and ULK1 which regulate autophagy initiation.
These proteins link to neurodegenerative diseases and cancer. Ubiquitin and related pathways play a role in conditions like Parkinson’s disease where impaired protein degradation is a factor. Altered expression of p62 or ATG16L1 can contribute to cancer progression through dysregulated autophagy. Both proteins interact with pathways affecting diseases with aberrant ubiquitin signaling or p62 accumulation associated with pathological states.
