Description
Key facts
Reacts with:Human,
Target:CD274See target data,
Applications:mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.
Product details:
PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK) is specifically designed and validated for multiplex analysis of the tumor microenvironment (TME) using tyramide signal amplification (TSA) on human formalin fixed paraffin-embedded tissue (FFPE) sections. The order of staining and antibody dilutions have been optimized on human tonsil FFPE tissue.
This panel empowers researchers to explore the spatial interactions of these important immuno-oncology biomarkers, immune checkpoint proteins and immune cell-phenotyping markers within the tumor micro-environment. It allows for the 6 targets to be fluorescently detected on the same tissue section, plus a nuclear counterstain (DAPI).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT, Akoya Biosciences®). Please refer to this kit's protocol for directions on how to perform the immunostaining.
The panel contains enough reagents for a minimum of 50 slides.
Panel contents, recommended order of staining and dilutions:
1. Recombinant PD1 antibody [CAL20]
ab237728
(50 μl)
1/500 dilution; Opal™520
2. Recombinant PD-L1 antibody [CAL10]
ab237726
(50 μl)
1/500 dilution; Opal™540
3. Recombinant CD68 antibody [SP251]
ab192847
- Macrophage marker (50 μl)
1/300 dilution; Opal™570
4. CD3 antibody [SP7]
ab16669
- T cell marker (50 μl)
1/300 dilution; Opal™620
5. Recombinant Ki67 antibody [SP6]
ab16667
- Proliferation marker (50 μl)
1/200 dilution; Opal™650
6. Pan-cytokeratin antibody [C-11]
ab7753
- Epithelial tissue marker (50 μg)
1/200 dilution; Opal™690
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
