Description
Key facts
Detection method:Colorimetric,
Assay type:Enzyme activity (quantitative),
Assay Platform:Microplate
Product details:
We recommend ATPase Assay Kit
ab234055
as a replacement for this kit. The assay mechanism in
ab234055
is identical to this assay kit; it is formulated with less assay components for greater convenience, and includes an ATPase enzyme positive control.
ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLock™ gives a stable end-point signal and is not prone to precipitation. PiColorLock™ has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis.
NB: For samples with free Pi, please check the Inorganic Phosphate Binding Resin (ab270547)
Other Notes
This product is manufactured by Expedeon, an Abcam company. It was previously called ATPase Enzyme Assay Kit. Expedeon product code 601-0120 is the same as the 2 x 96 tests size of this product.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
