Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.58 pg/mL,
Range:11.72 - 750 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Exendin-4 ELISA Kit ab272192 is a rapid single-wash 90-min Sandwich ELISA to measure Exendin-4 in cell culture media. This SimpleStep sensitivity is 2.58 pg/mL.
How the assay works
Exendin-4 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Exendin-4 ELISA Kit ab272192 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Exendin-4 is a 39 amino acid peptide found in venom from the Gila monster Helicoderma suspectum. Exendin-4 is a member of the glucagon secretin family of peptide hormones and neuropeptides. The venom protein mimics the incretin hormone glucagon-like peptide 1 (GLP-1) and stimulates insulin synthesis and secretion, protects against beta-cell apoptosis in response to different insults, and promotes beta-cell proliferation. Exendin-4 also promotes satiety, reduces food intake, fat deposition and body weight. It also is known to inhibit gastric emptying. Exendin-4 interactions with GLP-1 receptor (GLP1R). induces hypotension that is mediated by relaxation of cardiac smooth muscle.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Exendin-4 also known as "exendine 4" or "exendin e 4" is a peptide with significant activity in glucose metabolism. The peptide originated from the saliva of the Gila monster Heloderma suspectum and has a molecular mass of approximately 4.2 kDa. It is expressed mainly in the pancreas but also found in other tissues. Exendin-4 acts as a potent agonist of the glucagon-like peptide-1 (GLP-1) receptor which is expressed in various parts of the body such as the brain and gastrointestinal tract.
Biological function summary
Exendin-4 enhances insulin secretion helping to regulate blood glucose levels effectively. It mimics the activity of GLP-1 which is involved in stimulating insulin release. Exendin-4 also slows gastric emptying and promotes satiety contributing to reduced food intake. Although the peptide does not form part of a larger complex its interactions directly affect multiple cellular processes relevant to metabolism.
Pathways
Exendin-4 participates in the insulin signaling and glucose homeostasis pathways. It influences insulin receptor substrate proteins thereby playing an important role in maintaining normal blood sugar levels. Exendin-4 modulates the activity of gluconeogenic enzymes connecting it to metabolic pathways involving energy balance and storage.
Exendin-4 shows relevance in treating type 2 diabetes and obesity. By enhancing insulin secretion and reducing appetite it provides therapeutic effects for individuals with diabetes. It shares functional characteristics with proteins like GLP-1 and insulin which are implicated in these metabolic disorders. Research continues to explore its potential benefits and application in managing these conditions more effectively.
