Description
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 34 pg/mL,
Range:78.13 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human BMI1 ELISA kit (ab272210) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BMI1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMI1 with 34 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
BMI1 (Polycomb Complex Protein BMI-1), also known as Polycomb group RING Finger Protein 4, is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, BMI1 regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2. Human BMI1 amino acids sequence is 97% identical to mouse BMI1 and is 95% identical to rat BMI1.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bmi1 also known as B-cell-specific Moloney murine leukemia virus integration site 1 is a member of the Polycomb group of proteins. It has an approximate molecular mass of 37 kDa. Bmi1 is expressed in many tissues including the brain bone marrow and hematopoietic cells. It functions as a transcriptional repressor. Bmi1 plays an important role in chromatin remodeling influencing gene silencing and regulation of developmental processes. Bmi1 ELISA assays are commonly used for quantifying its levels in various research applications.
Biological function summary
The functions of Bmi1 extend into maintaining stem cell renewal and differentiation. It acts as part of the Polycomb repressive complex 1 (PRC1) partnering with other proteins to regulate gene expression by altering chromatin structure. Bmi1 protein regulates cell proliferation and inhibits senescence by repressing the INK4a/ARF locus which encodes tumor suppressor proteins like p16INK4a and p14ARF.
Pathways
Bmi1 plays significant roles in processes such as the DNA damage response and the self-renewal of hematopoietic stem cells. It is involved in the modulation of the Wnt and Notch signaling pathways. Bmi1 protein interacts closely with proteins such as CBX2 and RNF2 within these pathways affecting the transcriptional landscape that governs cell fate decisions.
Bmi1 overexpression links to cancer particularly breast cancer and leukemia. In MCF7 breast cancer cells alterations in Bmi1 activity contribute to oncogenesis and tumor progression. Its dysregulation can connect to Myc a well-known oncogenic protein. Additionally Bmi1's influence in Cas9-based gene editing models highlights its potential in cancer therapy research. Bmi1 has also been implicated in neurodegenerative disorders with connections to proteins like p21CIP1 affecting neuronal survival and aging.
