Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 14.3 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human BD-3 ELISA kit (ab272471) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BD-3 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BD-3 with 14.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Defensins are cysteine rich cationic peptides known to exhibit strong antimicrobial and immune signaling activities. BD-3, encoded by the
DEFB103A
gene, is expressed in many epithelial cells and induced by cytokines or microbial stimuli. Studies shows that BD-3 may play an important role in the innate epithelial defense and wound healing.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
