Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Plasma, Cell culture extracts, Cell culture supernatant, Serum,
Assay type:Quantitative,
Range:1 - 250 mU/mL,
Assay Platform:Microplate
Product details:
alpha-Mannosidase Assay Kit (ab272519) is a nonradioactive, colorimetric alpha-Mannosidase assay is based on the cleavage of 4-nitrophenol from a synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Fast and sensitive:
Linear detection range (10 μL sample): 1 to 250 U/L for a 10 minute reaction.
Convenient and high-throughput:
Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-Mannosidase also known as α-mannosidase or MAN2A1 is an enzyme that plays an important role in the processing of glycoproteins. This enzyme catalyzes the hydrolysis of alpha-mannosidic linkages in oligosaccharides. It has a molecular mass of approximately 125 kDa. Alpha-Mannosidase is expressed in various tissues throughout the human body including the liver kidney and brain. Its activity is especially high in the lysosomal compartment of cells where it participates in the breakdown of glycoproteins.
Biological function summary
The enzyme participates in the modification of N-linked glycoproteins during their processing in the endoplasmic reticulum and Golgi apparatus. It acts as part of a complex process that ensures proper folding and function of glycoproteins. Efficient activity of alpha-Mannosidase is essential for the maturation of glycoproteins which impacts cell surface signaling and protein stability. It also interacts with other enzymes and molecular chaperones during glycoprotein assembly.
Pathways
Alpha-Mannosidase plays a role in the glycosylation pathway and degradation of glycoproteins in the lysosome. It is involved in the N-glycan degradation pathway where its action precedes the function of other enzymes like beta-galactosidase and sialidase. Its function aligns with various glycosidase enzymes enabling the complete hydrolysis of oligosaccharides. This enzyme's work is connected directly to the processes influencing cellular homeostasis and turnover of glycoproteins.
Enzyme malfunctions can lead to alpha-Mannosidosis and other glycoprotein storage diseases. Alpha-Mannosidosis is characterized by a deficiency in this enzyme leading to the accumulation of mannose-rich oligosaccharides in cells causing symptoms like intellectual disability and skeletal abnormalities. The relationship between alpha-Mannosidase and lysosomal proteins such as lysosomal alpha-glucosidase highlights its role in the development of these conditions. Abnormalities associated with the enzyme's pathway can also affect cellular communication and immune system function marking its important role in health and disease.
