Description
Key facts
Detection method:Colorimetric,
Assay type:Quantitative,
Assay Platform:Microplate
Product details:
ATPase/GTPase Assay Kit (ab272520) offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 - 660 nm).
High sensitivity
: detection of 0.007 U/L ATPase or GTPase activity.
Fast and convenient:
single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes.
Robust and amenable to HTS
: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of over 0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATPases and GTPases are enzymes essential for energy transfer within cells. ATPases facilitate the hydrolysis of ATP while GTPases do the same with GTP. Alternate names for these enzymes include ATP hydrolase and GTP hydrolase. ATPases usually have a mass in the range of 100-500 kDa depending on the specific protein. These proteins are found in various cellular locations including the mitochondria cytoplasm and membranes reflecting their importance across different cellular functions.
Biological function summary
Both ATPases and GTPases play key roles in cellular activities such as transport signal transduction and cell movement. For example ATPases are critical for muscle contraction and ion transport while GTPases regulate processes like cell growth and apoptosis. These enzymes often function as part of protein complexes. In the case of ATPase examples include the F1Fo ATP synthase complex. GTPases can be found in complexes with other proteins influencing signaling pathways.
Pathways
Both ATPases and GTPases play integral roles in cellular signaling and energy metabolism pathways. ATPase activity is central to the oxidative phosphorylation pathway which is essential for ATP synthesis. Important proteins connected to this pathway include cytochrome c oxidase. GTPase activity is important in the Ras signaling pathway with proteins like Ras itself being significant components. These pathways help coordinate cellular responses to environmental changes and are fundamental to cell survival and function.
Dysregulation of GTPases and ATPases associates with several conditions. Aberrant GTPase activity can contribute to cancer as seen with mutations in the Ras protein which affect cell proliferation. The malfunction of ATPase can lead to mitochondrial diseases like Leigh syndrome which involves defective energy production. In these diseases mutations in specific proteins within the related pathways such as NADH dehydrogenase for ATPase further illustrate the connection between these enzymes and pathological conditions.
