Description
Key facts
Detection method:Colorimetric,
Sample types:Other biological fluids,
Assay type:Quantitative,
Range:7 - 300 µg/dL
Product details:
Simple, direct and automation-ready procedure for measuring copper concentrations in a wide range of applications in research, drug discovery and environmental monitoring. This copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured at 359nm, is directly proportional to copper concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Sensitive and accurate.
Linear detection range 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM) copper in 96-well plate assay.
Simple and high-throughput
. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Improved reagent stability and versatility.
The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Copper is a trace element essential for various enzymatic reactions also known as a transition metal. It is an important component of several metalloproteins and enzymes such as cytochrome c oxidase with a molecular mass ranging from 6000 to 16000 Daltons for its various bound forms. Copper expresses in tissues throughout the body particularly in the liver brain and kidneys. Analytical tools like copper assay kits and copper stain histology identify and quantify its presence within biological samples.
Biological function summary
Copper is integral to processes like oxidative phosphorylation and free radical detoxification. It functions within multi-subunit enzyme complexes aiding electron transfer and free radical scavenging. Copper-dependent enzymes such as superoxide dismutase and lysyl oxidase require copper for activity showcasing its role in cellular defense mechanisms and connective tissue biogenesis. Copper measurement is critical to assess its biological impact on these processes.
Pathways
Copper is involved in mitochondrial respiration and the electron transport chain important parts of energy metabolism. Copper influences pathways by participating in the electron transfer as part of cytochrome c oxidase and regulating the activity of mitochondrial cytochromes. It is closely related to proteins such as ATP7A and ATP7B which manage its cellular distribution and export integral to maintaining copper homeostasis in metabolic pathways.
Copper imbalance can lead to conditions like Wilson's disease and Menkes disease. Excessive copper accumulation in organs causes Wilson's disease attributed to mutations affecting copper-transporting ATPase (ATP7B) proteins. Menkes disease results from deficient copper absorption linked to ATP7A protein issues highlighting copper's importance in metabolic disorders. Copper test kits and copper staining aid in diagnosing and understanding these pathologies.
