Description
Key facts
Detection method:Colorimetric,
Sample types:Food samples, Saliva, Urine, Cell culture media,
Assay type:Quantitative,
Range:0.04 - 2 %
Product details:
Ethanol Assay Kit (ab272531) is a simple, direct and automation-ready procedure for measuring ethanol concentration. This assay kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr
) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Sensitive and accurate:
Detection range 0.04 – 2% alcohol in 96-well plate assay.
Convenient and high-throughput:
The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a highthroughput 96-well plate assay for thousands of samples per day.
Versatility:
Assays can be executed in 96-well plate or cuvet.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ethanol also known as ethyl alcohol is a small volatile molecule with a molecular weight of 46.07 g/mol. Ethanol naturally occurs in the fermentation process of yeast and is present in alcoholic beverages. In cellular environments it acts ubiquitously affecting lipid membranes and interacting with various protein structures. Ethanol is not a protein but a small organic compound that exerts mechanical effects on cell membranes and proteins due to its lipophilic nature.
Biological function summary
Ethanol plays a significant role in central nervous system modulation and energy metabolism. It is not a part of a complex but affects neurotransmitter systems by interacting with ion channels and receptors such as GABA and NMDA receptors. The presence of ethanol in biological systems can disrupt normal neural signaling leading to altered mood and cognitive function which is often observed in alcohol consumption.
Pathways
Ethanol impacts several critical biological processes including the central nervous system and metabolic pathways. It influences the gamma-aminobutyric acid (GABA) pathway by enhancing inhibitory neurotransmission leading to sedative effects. Ethanol also participates in the metabolic pathways related to the conversion of alcohol to acetaldehyde and then to acetic acid involving enzymes such as alcohol dehydrogenase and aldehyde dehydrogenase. This metabolic process connects ethanol to energy production and lipid metabolism.
Ethanol consumption is closely linked to alcohol use disorders and liver disease. Chronic exposure to ethanol can lead to liver cirrhosis a condition marked by the progressive destruction of liver tissue. The interaction of ethanol with proteins like cytochrome P450 enzymes can exacerbate liver damage. Ethanol's role in altering GABA receptor activity is also associated with addiction and dependency as it enhances the inhibitory effects leading to the development of tolerance and withdrawal symptoms.
