Description
Key facts
Detection method:Colorimetric,
Assay type:Quantitative,
Range:10 - 2000 ng/mL,
Assay Platform:Microplate
Product details:
The Bovine Immunoglobulin G ELISA Kit (ab273152) provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgGs in cell culture supernatants and serums (CS, FCS, NBCS...) and contaminating bovine IgGs in batches of purified antibodies produced in vitro. The kit includes ready-to-use reagents necessary to analyze 89 samples in less than 45 minutes.
A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Immunoglobulin G (IgG) often referred to as IgG is the most common type of antibody found in blood circulation. It is an important element of the immune response enabling the body to identify and neutralize pathogens such as bacteria and viruses. IgG antibodies have a molecular weight of approximately 150 kDa. They are produced by B cells and are distributed predominantly in blood and extracellular fluid allowing them to play a significant role in immunity. IgG antibodies come in four subclasses: IgG1 IgG2 IgG3 and IgG4 each differing in their heavy chain structure and effector functions.
Biological function summary
IgG antibodies function as an important component of the adaptive immune system. They form part of complex immune responses where they help in antigen recognition and neutralization. These antibodies can opsonize pathogens making them more recognizable to phagocytes for destruction. IgG also activates the complement system which contributes to the lysis of pathogenic cells. As a bridge between innate and adaptive immunity IgG mediates the interaction with natural killer (NK) cells enhancing the cell-mediated immune response.
Pathways
The signaling pathways involving IgG antibodies include the classical complement pathway and Fc receptor signaling. The classical complement pathway complements the antibodies in opsonizing pathogens promoting inflammation and leading to the lysis of pathogens. Fc receptors on immune cells recognize and bind to the Fc region of IgG triggering phagocytosis and the cytotoxic activity of immune effector cells. IgG is related to other proteins such as complement proteins and Fcγ receptors which are essential for its role in immune signaling.
Elevated or diminished levels of IgG are associated with conditions like autoimmune diseases and immunodeficiencies. For example rheumatoid arthritis can involve abnormal IgG response where alterations in Fc glycosylation impact its function connecting IgG to the disorder. In immunodeficiencies such as common variable immunodeficiency (CVID) patients may have low levels of IgG leading to increased susceptibility to infections. IgG in these diseases interacts with proteins like cytokines and immune receptors influencing disease progression.
