Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Dog,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.4 ng/mL,
Range:0.41 - 100 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Canine IL-2 ELISA Kit ab273172 is a sandwich ELISA to measure Canine IL-2 in serum, plasma, cell culture supernatant with a sensitivity of 0.4 ng/ml.
How the assay works
This assay employs an antibody specific for Canine IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Canine IL-2 ELISA Kit ab273172 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
