Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Cow,
Assay type:Sandwich (quantitative),
Sensitivity:= 16 pg/mL,
Range:16.38 - 4000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Bovine IL-1 alpha ELISA Kit (ab273196) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of bovine IL-1 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for bovine IL-1 alpha coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- bovine IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
