Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Cow,
Assay type:Quantitative,
Sensitivity:= 2 pg/mL,
Range:2.05 - 500 pg/mL,
Assay Platform:Microplate
Product details:
Bovine IL-17A ELISA Kit (ab273201) is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of bovine IL-17A in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for bovine IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti bovine IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
