Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Cat,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.16 ng/mL,
Range:0.16 - 40 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Feline IL-12p40 ELISA Kit (ab273231) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Feline IL-12p40 in serum, plasma and cell culture supernatants (feline IL-12p40 concentration is low in normal serum and plasma samples and may not be detectable in this assay).
This assay employs an antibody specific for feline IL-12p40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12p40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-feline IL-12p40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12p40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
