Description
Key facts
Detection method:Colorimetric,
Sample types:Suspension cells, Adherent cells, Cell Lysate,
Assay type:Quantitative,
Assay Platform:Microplate
Product details:
Caspase-1 Assay Kit (Colorimetric) (ab273268) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.
This product is manufactured by BioVision, an Abcam company and was previously called K111 Caspase-1 Colorimetric Assay Kit. K111-25 is the same size as the 25 test size of ab273268.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
