Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Cell culture media, Other biological fluids, Adherent cells,
Assay type:Quantitative,
Assay Platform:Microplate
Product details:
Asparagine Assay Kit (Fluorometric) (ab273333) allows for highly sensitive quantification of L-asparagine levels in biological fluids and cultured cells.
In the assay, L-asparagine is hydrolyzed to generate aspartate, which is converted to pyruvate and subsequently reacts with a fluorogenic probe to form a stable fluorophore (Ex/Em = 538/587 nm) via a series of coupled enzymatic reactions. The assay is not affected by physiological concentrations of other common amino acids and can detect asparagine levels down to 5 μM in samples.
This product was previously called K736 Biovision Asparagine Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Asparagine often referred to by its alternate names such as l-asparagine or aspargine is a non-essential amino acid. It plays an important role in the biosynthesis of glycoproteins and also acts as a building block of proteins. L-asparagine weighs approximately 132.12 g/mol. This amino acid is widely expressed in both prokaryotic and eukaryotic organisms appearing in high concentrations in tissues with significant protein synthesis activity like the liver and brain. It is known for its involvement in nitrogen transport and storage.
Biological function summary
Asparagine supports protein synthesis and is involved in metabolic regulation. It is significant for the synthesis of glycoproteins and other proteins. As part of its biological roles asparagine functions closely with another key amino acid glutamine which assists in the regulation of cellular pathways and amino acid synthesis. While asparagine itself is not part of a protein complex its synthesis and conversion are tightly regulated by the enzyme asparagine synthetase ensuring proper cellular function.
Pathways
Asparagine is integrated into protein synthesis and amino acid metabolism. These pathways interlink with the urea cycle where asparagine's contribution to nitrogen transfer becomes essential. One of the key proteins associated with these pathways is asparaginase which serves as a therapeutic enzyme in treatments of certain cancers. Furthermore asparagine interacts with enzymes within glycoprotein biosynthesis influencing protein folding and stability processes.
Asparagine is associated with conditions such as acute lymphoblastic leukemia (ALL) and neurodegenerative disorders. The enzyme asparaginase important in breaking down asparagine is used in treating ALL by depriving cancer cells of asparagine which they cannot synthesize independently. Additionally aberrant glycosylation of proteins involving asparagine has been linked to some neurodegenerative disorders emphasizing its importance in brain function and pathology.
