Description
Key facts
Detection method:Colorimetric,
Sample types:Serum,
Reacts with:Human,
Assay type:Quantitative,
Range:1.37 - 1000 U/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
COVID-19 S-Protein (S1RBD) Human IgG ELISA Kit (ab274340) is an
in vitro
indirect ELISA for the quantitative measurement of human IgG antibody against SARSCoV-2 S1 RBD protein in human serum.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 S1 RBD protein, which combines with the corresponding antibody present in a sample and Positive Control, which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of COVID19 S1 RBD protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Controls are from an inactivated serum sample which contains SARS-COV-2 S1 RBD protein human IgG antibody. We do not know the exact amount of SARS-COV-2 S1 RBD protein human IgG antibody in the Positive Control sample. The Positive Control can be used as a calibration curve for interpretation purposes in different assays.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The COVID-19 Spike Protein often referred to as SARS-CoV-2 Spike Protein is an important structural protein of the virus with a molecular mass around 180 kDa. This protein facilitates viral entry into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor which is primarily expressed in the respiratory tract. It's a trimeric protein composed of S1 and S2 subunits. The S1 subunit contains the receptor binding domain while the S2 subunit mediates membrane fusion.
Biological function summary
The spike protein plays an important role in viral infectivity and host range determination. The spike protein is part of the larger viral particle complex that includes other structural proteins like the nucleocapsid protein. Its interaction with host cell receptors triggers conformational changes allowing viral entry and subsequent replication within the host cells. This process is pivotal for the virus's lifecycle.
Pathways
The spike protein is integral to the viral entry pathway a critical step in the viral lifecycle. It intersects with the renin-angiotensin system (RAS) pathway through its interaction with the ACE2 receptor. This interaction not only facilitates viral entry but can also dysregulate the RAS pathway. Additionally the spike protein's activity is related to other viral proteins that assist in replication and assembly such as the envelope protein.
The spike protein is directly linked to COVID-19 the disease caused by SARS-CoV-2 infection. Its interaction with the ACE2 receptor has been associated with severe respiratory illnesses and potentially other conditions like cardiovascular disorders due to its effect on the RAS pathway. The nucleocapsid protein is also important in the virus's pathogenesis working together with the spike protein to establish infection and disease progression.
