Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Milk, Serum,
Reacts with:Cow,
Assay type:Sandwich (quantitative),
Range:3.13 - 200 ng,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Bovine Lactoferrin ELISA Kit ab274406 is a sandwich ELISA to measure Bovine Lactoferrin in serum, plasma, urine, milk.
How the assay works:
The Bovine Lactoferrin ELISA kit (ab274406) is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lactoferrin (LF) in bovine biological samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Bovine Lactoferrin ELISA Kit ab274406 protocol summary
1. Anti-LF antibodies bound to solid phase
2. Standards and Samples added
3. LF * anti-LF complexes formed
4. Unbound Sample proteins removed
5. Anti-LF-HRP Conjugate added
6. Anti-LF-HRP * LF * anti-LF complexes formed
7. Unbound anti-LF-HRP removed
7. Chromogenic Substrate added
8. Determine bound enzyme activity
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lactoferrin also known as lactotransferrin is an iron-binding glycoprotein with a molecular weight of about 80 kDa. It predominantly exists in mammalian milk particularly in high concentrations in colostrum and is also found in various epithelial secretions such as saliva tears and nasal fluids. The liver and neutrophils also produce lactoferrin and it plays a significant role in the body's primary defense system. As an antibacterial agent lactoferrin sequesters free iron to limit bacterial growth and disrupt biofilms assisting in the protection against microbial invasions.
Biological function summary
Lactoferrin exhibits multiple functions beyond its antimicrobial properties. It contributes to immunomodulation by binding to specific receptors on immune cells enhancing phagocytosis and influencing cytokine production. Also it possesses anti-inflammatory and antioxidant properties. Lactoferrin interacts with cellular components including glycosaminoglycans and lipoproteins impacting the activity and expression of proteins within signaling and metabolic pathways. This multifaceted role places it as a critical participant in both innate and adaptive immune responses although it does not form part of a stable protein complex.
Pathways
Lactoferrin plays a significant role in the iron metabolism and immune response pathways. It tightly interacts with the transferrin receptor modulating iron uptake and distribution across tissues which helps prevent iron overload and oxidative stress. Furthermore lactoferrin links with the toll-like receptor pathways particularly TLR4 which enables the modulation of innate immune responses against pathogens. By influencing nuclear factor kappa B (NF-kB) signaling lactoferrin impacts inflammation and immune responses working together with proteins such as ferritin and hepcidin.
Lactoferrin has relevancy to diseases like anemia of chronic disease and inflammatory bowel disease (IBD). Its role in regulating iron homeostasis makes it essential for preventing anemia associated with chronic inflammation. Lactoferrin mitigates dysregulated immune responses in IBD potentially reducing inflammation and promoting gut health. Disease-modifying properties of lactoferrin connect it to glycoproteins like transferrin and inflammatory mediators demonstrating its potential therapeutic importance.
