Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Mutation description:63 bp deletion (allele 1) and 1 bp insertion after Lys168 (allele 2) of the WT protein, Knockout.,
Disease:Adenocarcinoma
Product details:
Recommended control:
Human wild-type HeLa cell line (ab271142). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-TERT, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Telomerase reverse transcriptase also known as TERT is an enzyme with a molecular mass of about 127 kDa. It functions as a catalytic subunit of the telomerase complex which extends telomeres by adding the nucleotide sequence "TTAGGG" to the ends of chromosomes. TERT operates with a telomerase RNA component (TERC) to synthesize DNA using an RNA template a process called reverse transcription. TERT is expressed in embryonic tissues adult stem cells and cancer cells where rapid cell division occurs.
Biological function summary
TERT plays an important role in maintaining telomere length essential for protecting chromosome stability. The protein forms part of the telomerase holoenzyme complex working together with TERC to perform its function. Telomerase activity allows cells to bypass the Hayflick limit preventing senescence and enhancing replication potential. In addition to telomere maintenance TERT influences gene regulation and cellular proliferation.
Pathways
TERT holds significance in the telomere maintenance and signaling pathways. Telomere maintenance ensures genomic integrity and cellular longevity a process fundamental for tissue homeostasis and regeneration. TERT interacts with proteins such as dyskerin and TERC within these pathways. Its regulatory roles in these pathways also reveal interactions with other key proteins like hypothesized targets in the DNA damage response.
The upregulation of TERT is associated with cancer and degenerative diseases. Increased telomerase activity is a common feature in many cancers leading to uncontrolled cell proliferation. Additionally mutations in TERT and its associated components can contribute to disorders like dyskeratosis congenita a rare genetic condition resulting in bone marrow failure. In these conditions TERT interaction with proteins involved in chromosomal maintenance and repair becomes important for understanding disease mechanisms.
