Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 6.7 pg/mL,
Range:39 - 2500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Caspase 4 ELISA kit (ab275098) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Caspase 4 protein in cell culture and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Caspase 4 with 6.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Caspase-4 also known as ICE and Ced-3 homolog 2, Mih1, and protease TX, is a protein in the family of cysteine proteases called caspases and is encoded by the gene CASP4. CASP4 is expressed as an inactive proenzyme comprised of a pro-domain and a large and small protease subunit. CASP4 is activated by cleavage of an internal Aspartic residue, thus generates a heterodimeric enzyme consisting of the two subunits which is capable of cleaving and activating its own precursor protein. Overexpression of CASP4 results in apoptosis. The antibodies in this kit were generated using an immunogen sequence present in the large subunit (subunit 1).
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
