Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.92 ng/mL,
Range:1.88 - 120 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Asprosin ELISA kit (ab275108) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Asprosin protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Asprosin with 0.92 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Asprosin is a hormone that targets the liver to increase plasma glucose levels. Asprosin is secreted by white adipose tissue and circulates in the plasma. Asprosin acts in response to fasting and promotes blood glucose elevation by binding to the surface of hepatocytes. It promotes hepatocyte glucose release by activating the protein kinase A activity in the liver, resulting in rapid glucose release into the circulation. Asprosin and Fibrillin-1 are encoded by single FBN1 gene, they are translated and secreted as single precursor protein. Upon secretion these two proteins are separated by furin.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
