Description
Key facts
Target:pCambia1301See target data,
Form:LyophilizedSee storage information,
Storage buffer:Constituents: 71% Tris, 17% EDTA
Product details:
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the
reporter gene.
The pCambia1301 vector offers:
High copy number in
E.coli
for high DNA yields
pVS1 replicon for high stability in
Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with hygromycin B
Simple means to construct translational fusions to
reporter genes
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV
S promoter and terminated by the CaMV
S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.
This vector contains a fully functional
reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses
E.coli gusA
with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual
A.tumefaciens
cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the
gene and insert their own gene of interest in its place or use this vector to create fusions of
with their gene of interest. The vector contains the pUC18 polylinker-
Genbank Sequence data
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (
aphIII
nptIII
/3'5''-aminoglycoside phosphotransferase typeIII from
Enterococcus faecalis
pJH1) in bacteria: use 50 μg/mL in
E.coli
A.tumefaciens
In plant system:
Resistance Gene
(From a
Klebsiella
species, via
E.coli
Transformed plant selection antibiotic
Hygromycin B
Concentration in
Arabidopsis
50 μg/mL in germination medium
Concentration in Rice
50 μg/mL in 2N6 then in RGH6
Concentration in Tobacco
50 μg/mL in RMOP then in MST
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
