Description
Key facts
Target:pCambia2300See target data,
Form:LyophilizedSee storage information,
Storage buffer:Constituents: 71% Tris, 17% EDTA
Product details:
Vector contains genes for bacterial and plant selection with kanamycin and and no reporter gene to allow construction of user's own system.
The pCambia2300 vector offers:
High copy number in
E.coli
for high DNA yields
pVS1 replicon for high stability in
Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with kanamycin
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV
S promoter and terminated by the CaMV
S polyA signal.
This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes.
The pUC18 polylinker within the
fragment allows blue/white screening of clones in
E.coli
cloning work.
Genbank Sequence data
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (
aphIII
nptIII
/3'5''-aminoglycoside phosphotransferase typeIII from
Enterococcus faecalis
pJH1) in bacteria: use 50 μg/mL in
E.coli
A.tumefaciens
In plant system:
Resistance Gene
nptII/aph(3')II
from Tn5
Transformed plant selection antibiotic
Kanamycin
Concentration in
Arabidopsis
20 - 50 μg/mL
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
